CRG Symposium 2015 » Session 1

Resolving the building blocks and assembly principles of cell adhesion machineries

Jamina — Thu, 22 Oct 2015 11:15:02 +0000

Short Talk by Eli ZAMIR, from theDepartment of Systemic Cell Biology, Max Planck Institute of Molecular Physiology, Dortmund DE, about how adhesion sites get self-assembled and function.

Adhesion sites contain hundred different proteins called integrin adhesome. This proteins interact with each other, self-organizing as diverse adhesion sites with distinct properties
Studies have been carried out about this self assembly, investigating the state of the integrin adhesome network in the cytosol and space and time relations with focal adhesions
Using FCCS and FRAP measurements, it was found that cytosol complexes correlate with the internal organization: integrin adhesome is extensively pre-assembled in the cytosol, forming multi-protein building blocks for adhesion sites that are combinatorially diversified, and they correlate with the structural and functional organization of proteins across focal adhesions.
Building blocks enter and exit this focal adhesions without being altered, preserving specifications and assembly logic. They are currently investigating the recruitment of specific multi-protein blocs to focal adhesions and its regulation to decipher this assembly logic.

Reconstituting mitotic spindle organization in artificial confinement

Jamina — Thu, 22 Oct 2015 08:14:25 +0000

The first talk of the day, by Marileen Dogterom from the Department of Bionanoscience in the Kavli Institute of Nanoscience, Delft University of Technology, NL, is about Reconstituting Mitotic Spindle organization

Different experiments in artificial confinement on microtubule dynamics, their pushing and pulling forces.
Independent experiments observing the effect on mitotic spindle organization of Dynein, ACE 1 and others, show how microtubule organization and tubulin change when adding or removing them.

The 14th CRG Symposium starts now

Jamina — Thu, 22 Oct 2015 07:37:49 +0000

The 14th CRG Symposium, Cellular Machineries starts now with Section 1, introduced by Sebastian Maurer from the CRG. 6 Speakers from all over the world will be talking about Mechanochemistry of Cell Biology during the morning.