Optimization of Codon translation rates via tRNA modifications maintains proteome integrity
Session 3 of the 14th CRG Sympossium features, among others, Danny NEDIALKOVA from the Max Planck Institute for Molecular Biomedicine, Max Planck Research Group for RNA Biology, from Münster DE, presenting a short talk about how optimization of codon translation rates via tRNA modifications maintains proteome integrity.
- Codon translation speed can impact protein folding and tRNA modifications stabilize codon-anticodon pairing
- U34 (wobble modified codon) modifications are required for fitness. They studied how modified U34 enhances codon translation rates in vivo:
- Elevated tRNA levels restores cellular fitness.
- U34 modifications promote efficient decoding in yeast and maintain protein homeostasis, while loss of them triggers proteotoxic stress and elicits proteins aggregation
- Metastable proteins aggregate in cells with unmodified U34 while elevated tRNA levels restore protein homeostasis
- U34 modifications promote efficient decoding in metazoans while U34 modification loss impairs proteostasis in metazoans, and triggers protein homeostasis failure